PerfeCTa SYBR Green SuperMix, Low ROX
PerfeCTa® SYBR® Green SuperMix, Low ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR on Applied Biosystems 7500 or Stratagene MX series of real-time PCR systems. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. This supermix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
- 2X concentrated master mixes with stabilized SYBR Green dye and exceptional temperature stability (≥30 days at 22°C) that withstand repetitive freeze-thaw (≥ 20X).
- Maximum SYBR Green dye load for robust optical signal with small amplicons. · Supports efficient vortexmixing with proprietary anti-foaming technology.
- Maximum assay sensitivity and target precision with highly modified Taq DNA polymerase and stringentultrapure, AccuStart™ II antibody hotstart technology.
- Easy-to-use with AccuVue™ plate loading dye and pre-blended passive reference dye.
Performance Data
Comparison to QuantiTect, GAPD
![PageW - Graph Quanta vs Qiagen SYBR** - Quanta vs Qiagen SYBR](https://www.genetargetsolutions.com.au/wp-content/uploads/2014/01/Perfecta-SYBR-Green-Supermix.jpg)
PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD
Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.
Features & Benefits
- Buffer optimization for SYBR® Green stability, ensuring reliable performance
- High yield, high sensitivity
- Highly specific amplification
- Improved reproducibility and assay precision
Real-Time PCR Instrument Compatibility
Contents
- Single-tube, 2X concentrated reagent containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
- AccuStart II Taq DNA Polymerase
- SYBR Green I dye
- Inert AccuVue dye
- Proprietary enzyme stabilizers and performance-enhancing additives.
- Titrated reference dye (if applicable).
Storage conditions
PerfeCTa® SYBR Green SuperMix, Low ROX is stable for 1 year when stored in a constant temperature freezer at -20˚C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Resources:
- 95056 PerfeCTa SYBR Green Supermix, Low ROX Protocol
- 95056 PerfeCTa SYBR Green Supermix, Low ROX MSDS
- SYBR SuperMixes and FastMixes Product Brochure
Ordering
Cat# 95056-100 | 100 x 50-µl rxns (2 x 1.25 ml) | |
Cat# 95056-500 | 500 x 50-µl rxns (10 x 1.25 ml) | |
Cat# 95056-02K | 2000 x 50-µl rxns (1 x 50 ml) |