3rd July 2024

PerfeCTa qPCR ToughMix, UNG

PerfeCTa qPCR ToughMix UNG is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust realtime qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.PerfeCTa qPCR ToughMix UNG contains all required reaction components, except primers, probe(s), and DNA template. Inclusion of uracil DNA glycosylase (UNG), and substitution of dTTP with dUTP, prevents amplification of carry-over contamination from previous dU-containing PCRs.A key component of PerfeCTa qPCR ToughMix UNG is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix UNG delivers exceptional performance with either fast or conventional PCR cycling protocols.

Comparison to Conventional Master Mixes

PerfeCTa qPCR ToughMix stands up to the challenge where other qPCR master mixes fall apart.  qPCR is performed on clean template and in the presence of PCR inhibitors with PerfeCTa ToughMix and three relevant competitor’s Master Mixes (Figure 1). Average CT values obtained with PerfeCTa ToughMix are unaffected by the presence of the PCR inhibitors while competitor’s mixes show signs of inhibition and in some cases fail to amplify.

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Real-Time PCR Instrument Compatibility

  • Roche LightCycler™ 480
  • Qiagen/Corbett Rotor-Gene® Q
  • Qiagen/Corbett Rotor-Gene® 6000
  • Qiagen/Corbett Rotor-Gene® 3000
  • Eppendorf Mastercycler® ep realplex
  • Eppendorf Mastercycler® ep realplex2 s
  • Cepheid SmartCycler®
  • Bio-Rad CFX96™
  • Bio-Rad CFX384™
  • Bio-Rad/MJ Opticon®
  • Bio-Rad/MJ Opticon 2
  • Bio-Rad/MJ Chromo4™
  • Bio-Rad MiniOpticon™
  • Illumina Eco qPCR
  • Thermo Scientific PikoReal Cycler
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Contents

  • 2X concentrated reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dUTP), hot-start DNA polymerase, uracil DNA glycosylase (UNG), and stabilizers.

Storage conditions

PerfeCTa qPCR ToughMix UNG is stable for 2 years when stored in a constant temperature freezer at 20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Ordering

Cat# 95138-250 250 x 20 µl rxns (2 x 1.25 ml)
Cat# 95138-012 1250 x 20 µl rxns (10 x 1.25 ml)
Cat# 95138-05K 5000 x 20 µl rxns (1 x 50 ml)