OmniCleave™ Endonuclease

• Removal of nucleic acids from cell lysates (reduction of viscosity) for improved handling and yield of protein preparations.
• Removal of trace contamination by nucleic acids in protein preparations.
• Removal of host DNA from phage preparations.
• Improved electrophoretic and chromatographic separation of proteins isolated from whole-cell lysates.

OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides (Fig. 1). OmniCleave Endonuclease has the same substrate specificity and yields the same products as Benzonase®, an enzyme derived from Serratia marcescens.

Benefits

• Digests all forms of DNA and RNA including single-stranded and double-stranded linear, circular, and supercoiled.
• Active within a broad range of conditions normally used in protein purification.
• Available in bulk quantities and high concentrations.

Unit Definition: One unit of OmniCleave Endonuclease converts 1.0 A₂₆₀ (~60 µg) of sonicated salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in 50 mM Tris-HCl (pH 8.0 at 25°C) and 1 mM MgCl₂.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5 at 25°C), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

Quality Control: OmniCleave Endonuclease is free of detectable protease activity.