MasterPure™ DNA Purification Kit for Blood Version II

Applications

• Purification of genomic DNA from whole blood or buffy coat for most molecular biology applications.

The MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat. With the optimized protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes (Table 1). The purified DNA contains both high- and low-molecular-weight genomic DNA which can be used directly as a template in PCR amplification reactions (Fig. 1), for digestion with restriction enzymes (Fig. 2), for Southern blotting, or for other molecular biology applications. Protocols are provided for genomic DNA and TNA purification from whole blood and buffy coat. For RNA samples, we recommend the MasterPure RNA Purification Kits. Both protocols allow for scaling up to accommodate larger samples.

Benefits

• Consistent yields of up to 10 µg DNA from 325 µl of whole blood and approximately 100 µg DNA from 600 µl of buffy coat.
• A₂₆₀/A₂₈₀ ratios of 1.8-2.0.
• Protocol can be completed in less than 30 minutes.
• No columns to reduce yields.
• No organic solvents are used.
• Purified DNA is PCR-ready and archive-quality.
• Scalable protocol for large or small samples.

Figure 1. Efficient multiplex PCR of purified blood DNA. A 100-ng aliquot of human genomic DNA purified with the MasterPure™ DNA Purification Kit for Blood Version II was amplified using the TAQXpedite™ PCR System with nine primer sets representing different exons of the dystrophin gene. All nine amplicons were produced in three blood DNA amplification reactions.	Figure 2. Efficient restriction endonuclease digestion of human genomic DNA purified from frozen whole blood with the MasterPure™ DNA Purification Kit for Blood Version II. A 1-µg aliquot of purified DNA was digested for 2 hours with each restriction endonuclease: M) Kilobase Ladder; 1) Bgl II; 2) EcoR I; 3) Hae III; 4) Hind III; 5) Pvu II; 6) Rsa I; 7) Xba I.

Table 1. Yields of DNA using the MasterPure™ DNA Purification Kit for Blood Version II.

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