Genotyping ToughMix

Features & Benefits

• Bullet-Proof Genotyping – easily overcome tough PCR inhibitors
• Short Run Times—fast cycling protocols enable more experiments
• Signal Optimization – robust genotyping with low primer & probe concentration
• Flexible Workflow – assembled reactions stable 3 days pre/post-PCR

Description

AccuStart Genotyping ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. AccuStart Genotyping ToughMix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.A key component of AccuStart Genotyping ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. AccuStart Genotyping ToughMix is compatible with both fast and conventional PCR cycling protocols.

Overcome PCR Troublemakers

Many sample types are rich in PCR inhibitors, see table below. These scoundrels operate by shutting down PCR amplification at threshold concentrations, or by quenching fluorescent signal. Expensive or time-consuming purification steps are no longer required to eliminate inhibitors – AccuStart Genotyping ToughMix neutralizes problem causing inhibitors in crude lysates and other difficult samples.

Inhibitor	Common Sources	Reagent Performance
		Competitor	AccuStart ToughMix®
Polyphenols	Plant Extracts
Humic Acids	Soil, Plant Tissue
Hematin	Dried Blood, Blood Spots
Hemoglobin	Blood
Polysaccharides	Feces, Plant Tissue
Melanin	Hair, Skin

Performance Data