Exo-Minus Klenow DNA Polymerase (D355A, E357A)

Note: This product replaces Exo-Minus Klenow DNA Polymerase (Cat Nos. KL0810250, KL081001K, KL105000, and KL080911K), which will be discontinued effective May 31, 2012.

Applications

• Random-primer labeling of DNA.¹
• Second-strand cDNA synthesis.²
• Strand-displacement amplification.³

Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and 3´→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).

Unit Definition: One unit of Exo-Minus Klenow DNA Polymerase (D355A, E357A) converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.

Quality Control: Exo-Minus Klenow DNA Polymerase (D355A, E357A) is free of detectable endo- and exonuclease and RNase activities.

References

1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.
2. Sanbrook, J. et al. (1989) In: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. v.2, p. 8.14.
3. Walter, N.G. (1995) J. Mol. Biol. 254, 856.

Ordering

Catalog No.	Concentration	Size