BACMAX™ DNA Purification Kit

Applications

• Isolation of exceptionally pure BAC DNA that can be used for many applications including sequencing, fingerprinting, PCR, and preparation of shotgun libraries.

The BACMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality BAC DNA. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6-25 µg of BAC DNA from 1.5-100 ml of a single-copy fosmid culture. Selective precipitation steps and the incorporation of Epicentre’s RiboShredder™ RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications.

The BACMAX Kit is ideal for the purification of induced BACs produced with CopyControl™ BAC Vectors. The CopyControl cloning technology enables the user to grow the BAC clones at single copy to ensure insert stability and cloning of toxic gene products and then induce the clones to high-copy number for high yields of DNA. Using the BACMAX Kit, up to 4 µg of BAC DNA can be isolated from 1.5 ml of an induced clone.

Benefits

• Simple protocols isolate up to 0.6, 15, or 25 µg of BAC DNA from 1.5-, 40- or 100-ml cultures of a single-copy BAC, respectively.
• The kit does not use columns or binding resins that lower yields and shear the DNA.
• Isolate the amount of DNA that is needed, without wasting kit reagents.
• Ideal for isolating DNA from single-copy BAC clones or induced CopyControl™ BAC clones.
• No toxic organic solvents.

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