AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit
Applications
- Production of nonradioactive RNA probes for any purpose, including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.
The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.
Benefits
- The kit uses Epicentre’s AmpliScribe T7-Flash rapid, high-yield in vitro transcription technology to generate the highest possible yield of biotin-RNA.
- In vitro transcription reaction conditions are optimized to yield optimal levels of uniformly labeled biotin-RNA for high signal intensity.
- RNase Inhibitor included.
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Table 1. Yield of Biotin-RNA (in mg) from varying amounts of control template DNA from a standard 37°C, 20 ml AmpliScribe T7-Flash Biotin-RNA Transcription Kit reaction over time. Results may vary depending on the template used.
Ordering
Catalog No. | Size |
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AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit
ASB71110 | 10 Reactions |
Contents: AmpliScribe™ T7-Flash™ Enzyme Solution, RiboGuard™ RNase Inhibitor, AmpliScribe™ T7-Flash™ 10X Reaction Buffer, NTP/Biotin-UTP PreMix (ATP, CTP, GTP, UTP, and Biotin-16-UTP) Solution, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized).