AccuStart II PCR SuperMix

AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Features & Benefits

• High yield, high sensitivity
• Precise amplification – hot-start technology ensures specific and efficient primer extension
• Superior reproducibility and precision
• Convenient reaction assembly at room temperature

Contents

• 2X reaction buffer containing 3 mM MgCl2
• 0.4 mM each dNTP (dATP, dCTP, dGTP, dTTP)
• AccuStart II Taq DNA Polymerase and stabilizers.

Storage conditions

AccuStart II PCR SuperMix is stable for 1 year when stored in a constant temperature freezer at -20?C. For convenience, it may be stored unfrozen at +2 to +8ºC for up to 12 months. Repeated freezing and thawing does not impair product performance.

Precautions and Disclaimer

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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