2´-Deoxyribonucleoside-5´-Triphosphate Solutions (dNTPs)

Sterile, neutral pH solutions of dATP, dCTP, dGTP, and dTTP are available either as individual nucleotides or a mixture of all four nucleotides for use in DNA synthesis. Solutions containing only one dNTP are available in 100 mM concentrations. Premixed solutions containing all four dNTPs are also available at two concentrations, 2.5 mM and 25 mM of each dNTP. Each solution is sufficient for at least 1,000 50-µl reactions at concentrations used in typical reactions with most DNA polymerases. A sterile, pH neutralized 20 mM solution of dUTP is also available for synthesis of uracil-containing DNA.

Storage Buffer: dNTP solutions are made with sterile deionized water and adjusted to pH 7.0.

Quality Control: All of Epicentre’s dNTP solutions meet spectrophotometric criteria and are function-tested using our DNA polymerases.

dATP (2´-Deoxyadenosine-5´-Triphosphate)
Formula (free acid): C10H16N5O12P3Molecular weight: 491
A250/A260 = 0.80
A280/A260 = 0.15
dCTP (2´-Deoxycytidine-5´-Triphosphate)
Formula (free acid): C9H16N3O13P3Molecular weight: 467
A250/A260 = 0.84
A280/A260 = 0.98
dGTP (2´-Deoxyguanosine-5´-Triphosphate)
Formula (free acid): C10H16N5O13P3Molecular weight: 507
A250/A260 = 1.15
A280/A260 = 0.66
dTTP (2´-Deoxythymidine-5´-Triphosphate)
Formula (free acid): C10H17N2O14P3Formula weight: 482
A250/A260 = 0.65
A280/A260 = 0.71
dUTP (2´-Deoxyuridine-5´-Triphosphate)
Formula (free acid): C9H15N2O14P3
Formula weight: 468A250/A260 = 0.75
A280/A260 = 0.35

Solutions of 2´-Fluorine-2´-deoxycytidine-5´-triphosphate (2´-Fluorine-dCTP) and 2´-Fluorine-2´-deoxyuridine- 5´-triphosphate (2´-Fluorine-dUTP) are also available.

Ordering

Catalog No. Concentration Size
Premixed dNTP Solutions (All 4)
D08104 2.5 mM 10 µmoles (4 ml)
D59104 25 mM 10 µmoles (400 µl)
Includes dATP, dCTP, dGTP and dTTP. Concentrations and sizes are for each dNTP.
dATP Solution
D5910A 100 mM 10 µmoles (100 µl)
dCTP Solution
D5910C 100 mM 10 µmoles (100 µl)
dGTP Solution
D5910G 100 mM 10 µmoles (100 µl)
dTTP Solution
D5910T 100 mM 10 µmoles (100 µl)
20 mM dUTP Solution
D1905U 20 mM 5 µmoles (250 µl)